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無菌性
sterile-filtered
品質水準
製品種目
BioReagent
フォーム
liquid
テクニック
cell culture | mammalian: suitable
不純物
endotoxin, tested
輸送温度
ambient
保管温度
2-8°C
関連するカテゴリー
詳細
SITE Liquid Media Supplement (100×) serves as a substitute for serum-free formulations. It contains purified factors required for in vitro growth and differentiation studies. The addition of supplements to media will vary, depending on the cell type being studied and the basal medium employed. It is a general cell supplement designed for use in non-complex media (e.g., minimum essential medium (MEM), Roswell Park Memorial Institute Medium (RPMI-1640)) and complex media (e.g., Ham′s F-12, Dulbecco′s Modified Eagle Medium (DME) /F-12, MEM) with sodium pyruvate.
アプリケーション
SITE Liquid Media Supplement (100×) has been used:
- in the 3D culture of mouse embryonic pancreatic epithelial cells
- to isolate rabbit gastric glands and parietal cells
- to isolate rabbit single proximal tubule cells
生物化学的／生理学的作用
- Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
- Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum.
- Selenium: an essential trace element normally provided by serum.
- Ethanolamine: plays a significant role in the proliferation of hybridoma cells and frequently is added to supplements used for culturing these cells.
構成
1.0 mg/mL組み換えヒトインスリン、0.55 mg/mLヒトトランスフェリン(実質的に鉄不含)、0.5 μg/mL亜セレン酸ナトリウム、0.2 mg/mLエタノールアミンを含有します。
調製ノート
フェノールレッドを含まないアール平衡塩類溶液(EBSS)で調製されています。
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
S4920-PH:
S4920-BULK:
S4920-5ML:
S4920-VAR:
S4920PROC:
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