RAB0178

Human Fas Ligand ELISA Kit

for serum, plasma, and cell culture supernatants

• 別名: FasL
• UNSPSCコード: 41116158
• NACRES: NA.32

特性

• 化学種の反応性: human
• 包装: kit of 96 wells (12 strips x 8 wells)
• テクニック: ELISA: suitable; capture ELISA: suitable
• 入力: sample type plasma; sample type serum; sample type cell culture supernatant(s)
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 2 pg/mL; standard curve range: 1.37-1000 pg/mL
• 検出方法: colorimetric
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: human ... FASLG(356)

詳細

詳細

The Human Fas Ligand ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Fas Ligand in serum, plasma, cell culture supernatants and urine.

免疫原

Recombinant Human Fas Ligand

アプリケーション

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

生物化学的／生理学的作用

Binding of Fas Ligand (FasL) to Fas receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activate B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD (Fas-associated protein with death domain) followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to eventual cell death. FasL is also involved in AGE (advanced glycation end-product)-mediated apoptosis in human retinal ARPE-19 cells, suggesting its role in diabetic retinopathy. Changes in the activity of FasL suppress normal apoptosis, leading to abnormal survival and growth of tumor cells. Mutations in the FasL gene causes autoimmune lymphoproliferative syndrome (ALPS).

その他情報

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

キットの構成要素は別途購入可能です。

• RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

安全性情報

• ピクトグラム: GHS05
• シグナルワード: Warning
• 危険有害性情報: H290
• 注意書き: P234 - P390
• 危険有害性の分類: Met. Corr. 1
• 保管分類コード: 8A - Combustible corrosive hazardous materials