おすすめの製品
由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
Rab5-65, monoclonal
形状
buffered aqueous solution
分子量
~24 kDa
化学種の反応性
human, cat, bovine, mouse, rat
濃度
~2 mg/mL
テクニック
immunocytochemistry: 5-10 μg/mL using HeLa cells or NIH-3T3 or NRK
indirect ELISA: suitable
microarray: suitable
western blot: suitable
アイソタイプ
IgG3
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... RAB5A(5868)
mouse ... Rab5a(271457)
rat ... Rab5a(64633)
詳細
Anti-Rab5 antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the hybridoma Rab5-65 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to C-terminal region of human Rab5, conjugated to KLH. Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane.
Rab5 (Ras-related protein Rab-5A) is a small GTPase , that has three isoforms - Rab5A, Rab5B and Rab5C. It is upregulated in several secretory cells. It is located on human chromosome 3p24.
免疫原
synthetic peptide corresponding to the C- terminal region of human Rab5, conjugated to KLH.
アプリケーション
Anti-Rab5 antibody, Mouse monoclonal has been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
- co-immunoprecipitation assay
- western blotting
- immunofluorescence
生物化学的／生理学的作用
Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and early endosome antigen 1(EEA1).
Rab5 (Ras-related protein Rab-5A) controls endosomal trafficking. Overexpression of Rab5A leads to metastasis and poor prediction in colorectal cancer patients. It modulates common Rab5 effectors to control the secretory pathways. It controls the homotypic integration between early endosomes and also the integration between endocytic vesicles and early endosomes.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
The Autophagic Process Occurs in Human Bone Metastasis and Implicates Molecular Mechanisms Differently Affected by Rab5a in the Early and Late Stages
Maroni P, et al.
International Journal of Molecular Sciences, 17(4) (2016)
Probing the druggability of membrane-bound Rab5 by molecular dynamics simulations
Edler E and Stein M
Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 434-443 (2017)
Pamela A Godoy et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 142, 111968-111968 (2021-08-04)
Amyloid beta peptide (Aβ) is tightly associated with the physiopathology of Alzheimer's Disease (AD) as one of the most important factors in the evolution of the pathology. In this context, we previously reported that Aβ increases the expression of ionotropic
Prolonged morphine treatment alters delta opioid receptor post-internalization trafficking
Ong EW, et al.
British Journal of Pharmacology, 172(2), 615-629 (2015)
Missing-in-metastasis protein promotes internalization of magnetic nanoparticles via association with clathrin light chain and Rab7
Zhao P, et al.
Biochimica et biophysica acta. General subjects, 1863(2), 502-510 (2019)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）