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buffered aqueous glycerol solution
濃度
10,000 units/mL
輸送温度
wet ice
保管温度
−20°C
特異性
認識配列:5'-C/C(A,T)(T,A)GG-3'
ライゲーション・再切断アッセイ:2～10倍量のSty I で1 μgのλDNA基質を過剰消化した場合、切断率は100%、ライゲーション率は>95%、再切断率は>95%です。
熱失活:65°C、15分間で失活します。
ライゲーション・再切断アッセイ:2～10倍量のSty I で1 μgのλDNA基質を過剰消化した場合、切断率は100%、ライゲーション率は>95%、再切断率は>95%です。
熱失活:65°C、15分間で失活します。
その他情報
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
物理的形状
20 mM Tris-HCl溶液(pH 7.5、0.1 mM EDTA、50 mM KCl、10 mM 2-メルカプトエタノール、0.1%ゼラチン(v/v)、0.01%ポリドカノール(v/v)、50%グリセロール(v/v)含有、4°C)
K Mise et al.
Gene, 33(3), 357-361 (1985-01-01)
A new restriction endonuclease, StyI, free of contaminating nuclease activities, has been isolated from Escherichia coli carrying the hsd+ miniplasmid of Salmonella typhi origin. In the presence of 10 mM Mg2+, it recognizes and cleaves a hexanucleotide sequence of 5'-C
C Kessler et al.
Gene, 47(1), 1-153 (1986-01-01)
The properties and sources of all known restriction endonucleases and methylases are listed. The enzymes are cross-indexed (Table I), classified according to their recognition sequence homologies (Table II), and characterized within Table II by the cleavage and methylation positions, the
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential
Annabel A Ferguson et al.
Methods in molecular biology (Clifton, N.J.), 940, 87-102 (2012-10-30)
The generation of transgenic animals is an essential part of research in Caenorhabditis elegans. One technique for the generation of these animals is biolistic bombardment involving the use of DNA-coated microparticles. To facilitate the identification of transgenic animals within a
TALENs and ZFNs are associated with different mutation signatures.
Yongsub Kim et al.
Nature methods, 10(3), 185-185 (2013-02-12)
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