R6878

モノクロナール抗リン酸化-網膜芽細胞腫 (pSer⁷⁹⁵) マウス宿主抗体

~2 mg/mL, clone RB-10, purified immunoglobulin, buffered aqueous solution

• 別名: 抗Rb, リン酸化型(pSer⁷⁹⁵)抗体
• MDL番号: MFCD03457570
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: RB-10, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen 110 kDa
• 化学種の反応性: mouse, rat, human, bovine, hamster
• 濃度: ~2 mg/mL
• テクニック: immunohistochemistry: suitable using 3% paraformaldehyde fixation, 0.15% Triton X-100 permeabilization; indirect ELISA: suitable; microarray: suitable; western blot: 2-4 μg/mL using a whole cell extract of human melanoma cell line G-361
• アイソタイプ: IgG1
• UniProtアクセッション番号: P06400
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: phosphorylation (pSer795)
• 遺伝子情報: human ... RB1(5925) ; mouse ... Rb1(19645) ; rat ... Rb1(24708)

詳細

詳細

Monoclonal Anti-phospho-Retinoblastoma (Rb) (pS795) (mouse IgG1 isotype) is derived from the RB-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic phospho peptide corresponding to the C-terminal amino acids of human retinoblastoma protein, conjugated to KLH. Retinoblastoma proteins p107, p110 (also designated Rb, or pRb) and p130, referred to collectively as pocket proteins, constitute a nuclear protein family that share a common structural unit (the pocket) dedicated to binding certain transcription factors and thereby regulating cellular proliferation. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with conserved LXCXE motif.

免疫原

ヒト網膜芽細胞腫タンパク質のC末端アミノ酸791~804(pSer⁷⁹⁵)に相当する合成リン酸化ペプチドのKLH結合体。

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Monoclonal Anti-phospho-Retinoblastoma (pSer⁷⁹⁵) antibody produced in mouse has been used in:

• immunoblotting
• immunocytochemistry
• immunofluorescence
• fluorescence-activated cell sorting (FACS) assays
• enzyme linked immunosorbent assay (ELISA)

生物化学的／生理学的作用

Retinoblastoma (Rb) exerts its function by repressing the transcription of cellular genes required for DNA replication and cell division. It can be phosphorylated at multiplicity of sites in vitro by cell division cycle protein 2 (Cdc2), cyclin-dependent kinase (Cdk2) and Cdk4/Cdk6 kinase complexes. Phosphorylation of pS⁷⁹⁵ is required to inactivate Rb imposed growth suppression.

物理的形状

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids