おすすめの製品
由来生物
mouse
結合体
unconjugated
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
Rab9-29, monoclonal
形状
buffered aqueous solution
分子量
~23 kDa
化学種の反応性
human, rat, hamster, canine, mouse
濃度
~2 mg/mL
テクニック
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 2 μg/mL using total cell extract of HeLa cells.
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... RAB9A(9367)
mouse ... Rab9(56382)
rat ... Rab9a(84589)
詳細
Monoclonal Anti-Rab9 (mouse IgG1 isotype) is derived from the hybridoma Rab9-29 produced by the fusion of mouse cells (NS1 cells) and splenocytes from BALB/c mice. Ras-related protein Rab-9A (Rab9) is a member of the Rab family of small guanosine triphosphatases (GTPases). The gene Rab9 is mapped to human chromosome Xp22.2. Rab9 GTPase is localized predominantly to late endosomes.
特異性
Monoclonal Anti-Rab9 recognizes human, canine, rat, mouse and hamster Rab9 (approx. 23 kDa)
免疫原
ヒトRab9のアミノ酸187-200に相当する合成ペプチドのKLH結合体。
アプリケーション
Monoclonal Anti-Rab9 antibody produced in mouse has been used in:
- western blotting
- enzyme-linked immunosorbent assay(ELISA)
- immunoprecipitation
- immunocytochemistry
生物化学的／生理学的作用
Ras-related protein Rab-9A (Rab9) guanosine triphosphatase (GTPase) is required for the transport of mannose 6-phosphate receptors (MRPs) from endosomes to the trans-Golgi network (TGN). Rab GTPases are central regulators of membrane trafficking between the different subcellular compartments of the eukaryotic cell. Rab9 GTPase in its GTP bound conformation binds specifically to an effector protein named p40 that stimulates the transport of mannose 6-phosphate receptors (MPRs) from endosomes to the TGN. Rab9 also interacts with the vesicle cargo selection protein tail-interacting protein of 47 kDa (TIP47). This interaction is required for Rab9 stability on late endosomes and may be crucial for vesicular transport in the virus life cycle.
物理的形状
0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。
保管および安定性
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our 製品選択ツール.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
R5404-VAR:
R5404-200UL:
R5404-BULK:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Daniel P Kloer et al.
The Journal of biological chemistry, 285(10), 7794-7804 (2010-01-06)
The Hermansky-Pudlak syndrome (HPS) is a genetic hypopigmentation and bleeding disorder caused by defective biogenesis of lysosome-related organelles (LROs) such as melanosomes and platelet dense bodies. HPS arises from mutations in any of 8 genes in humans and 16 genes
Daniela Ploen et al.
European journal of cell biology, 92(12), 374-382 (2014-02-01)
Hepatitis C virus (HCV) morphogenesis and release are closely linked to lipid metabolism. It has been described recently by our group that TIP47 plays an essential role for the targeting of the NS5A-complexed RNA genome from the replicon complex to
Ana Kucera et al.
Communicative & integrative biology, 9(4), e1204498-e1204498 (2016-08-31)
The small GTPase Rab9 has long been described as a protein that mediates endosome-to-trans-Golgi Network (TGN) transport, and specifically mannose-6-phospate receptor (MPR) recycling. However, studies have challenged this view by showing that Rab9 also is connected to sorting pathways toward
Laurie K Jackson et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(37), 14141-14146 (2008-09-13)
Salmonella's success at proliferating intracellularly and causing disease depends on the translocation of a major virulence protein, SifA, into the host cell. SifA recruits membranes enriched in lysosome associated membrane protein 1 (LAMP1) and is needed for growth of Salmonella
Tatiana Kaptzan et al.
The American journal of pathology, 174(1), 14-20 (2008-12-06)
Niemann-Pick, type C (NP-C) disease is an autosomal recessive neurovisceral storage disorder in which cholesterol and sphingolipids accumulate. There is no specific treatment for this disease, which is characterized by progressive neurological deterioration, sometimes accompanied by hepatosplenomegaly. We and others
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）