PCRISPR004

Mouse CRISPR Brie Knockout Library

• 別名: CRISPR Brie Library, CRISPR Library for Mice, Mouse Knockout Library
• UNSPSCコード: 41105904
• NACRES: NE.02

特性

• 包装: pkg of 5 vials (5x200µL aliquots )
• 濃度: ≥5x108 VP/ml (via p24 Assay)
• アプリケーション: CRISPR
• 輸送温度: dry ice
• 保管温度: −70°C

詳細

詳細

The mouse CRISPR "Brie" lentiviral pooled libraries are designed using optimized metrics, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018), which combine improved on-target activity predictions (Rule Set 2) with an off-target score, the Cutting Frequency Determination (CFD). The library is designed to be compact and efficient to maximize screening efficiency and performance.

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

アプリケーション

• Functional Genomics/Target Validation
• Unbiased wholed genome forward genetic screening
• Validated positive and negative controls
• Set up and optimization of screen assay

特徴および利点

• Focus on your research, and we will generate your lentivirus screening library.
• Use CRISPR nucleases to knockout protein-coding genes to assess their function.
• Mouse Genes targeted 19,674
• Compact library of ~ four gRNAs per gene (78,637 total)
• Total Controls 1000(pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO for sources of Cas9.

原理

In a CRISPR KO screen, Cas9 introduces double-strand breaks at locations specified by gRNA. When the endogenous non-homologous end-joining (NHEJ) DNA repair system corrects these breaks, this often leads to the introduction of frame-shift mutations that effectively knock out the gene. Thus, the power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease.

法的情報

CRISPR USE LICENSE AGREEMENT

LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable