P8996

モノクローナル抗PINCH-1抗体 マウス宿主抗体

clone PINCH-C58, purified immunoglobulin, buffered aqueous solution

• MDL番号: MFCD06798713
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ELISA (i); IP; WB
• クローン: PINCH-C58, monoclonal
• 化学種の反応性: hamster, bovine, mouse, monkey, canine, human, rat
• citations: 6
• テクニック: immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 1-2 μg/mL using total cell extract from 293T cells

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: PINCH-C58, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~37 kDa
• 化学種の反応性: hamster, bovine, mouse, monkey, canine, human, rat
• テクニック: immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 1-2 μg/mL using total cell extract from 293T cells
• アイソタイプ: IgG1
• UniProtアクセッション番号: P48059
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... LIMS1(3987) ; mouse ... Lims1(110829) ; rat ... Gcc2(309798)

詳細

詳細

Monoclonal Anti-PINCH-1 (mouse IgG1 isotype) is derived from the hybridoma PINCH-C58 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a peptide of human PINCH1, conjugated to KLH. LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) or particularly interesting new Cys-His protein 1 (PINCH-1) is widely expressed. It has five LIM domains. It has leucine-rich nuclear export signal (NES) and nuclear localization signal (NLS) at the C-terminus. Human PINCH-1 and 2 are encoded by two different genes that are 82% identical in their amino acid sequence.

免疫原

ヒトPINCH-1のアミノ酸281-299に相当するペプチドのKLH結合体。

アプリケーション

Monoclonal Anti-PINCH-1 antibody produced in mouse has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• co-immunoprecipitation

生物化学的／生理学的作用

The interaction with PINCH proteins (PINCH-1 and 2) is important for localization of integrin linked kinase protein to cell matrix contact sites. It plays a key role in cell−matrix adhesion. PINCH2 is co-expressed and co-localized with PINCH-1/ILK complex. PINCH1 interacts with Wilms tumor 1 protein (WT1) in podocytes. Elevated expression of PINCH1 is observed in human laryngeal carcinoma.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable