P8874

モノクロナール抗ホスファカン マウス宿主抗体

~2 mg/mL, clone 122.2, purified immunoglobulin, buffered aqueous solution

• 別名: Anti-PTPRB, Anti-Receptor-type Protein-tyrosine phosphatase β
• MDL番号: MFCD09265428
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 122.2, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~180 kDa (higher band may be present)
• 化学種の反応性: rat
• 包装: antibody small pack of 25 μL
• 濃度: ~2 mg/mL
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: 0.2-0.4 μg/mL using total extract of rat brain
• アイソタイプ: IgM
• UniProtアクセッション番号: Q62656
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: rat ... Ptprz1(25613)

詳細

詳細

Monoclonal Anti-Phosphacan (mouse IgM isotype) is derived from the hybridoma 122.2 produced by the fusion of mouse myeloma cells (P3X cells) and splenocytes from BALB/c mice immunized with rat brain proteoglycans. Chondroitin sulfate proteoglycans are neural cell adhesion molecules (NCAM) ligands present in the brain extracellular matrix (ECM). Phosphacan protein is expressed mainly in astrocytes and is a ligand for NCAM. Phosphacan is the soluble extracellular domain of the receptor-type transmembrane protein tyrosine phosphatase (RPTPb).

免疫原

rat brain proteoglycans.

アプリケーション

Monoclonal Anti-Phosphacan antibody produced in mouse has been used in:

• immunoblotting
• immunohistochemistry
• immunocytochemistry.

生物化学的／生理学的作用

Phosphacan levels elevates during late embryogenesis. It reaches a plateau two weeks postnatal before reaching stable. Receptor-type transmembrane protein tyrosine phosphatase (RPTPb) functions to promote primary tecal neurons neurite growth, neural migration and also induces cell adhesion. Both phosphacan and RPTPb can bind to NCAM and tenascin-C and −R. Phosphacan can oppose RPTPb by competing for its binding sites. Both in hippocampal and spinal cord neurons, phosphacan can affect neuronal adhesion and neurite outgrowth.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)