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Key Documents

安全性情報

P8045

Sigma-Aldrich

Protein G-Biotin from Streptococcus sp.

recombinant, expressed in unspecified host, lyophilized powder

別名:

Biotinylated Protein G, Protein G Biotin, Protein G-Biotin

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About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.46

リコンビナント

expressed in unspecified host

品質水準

結合体

biotin conjugate

形状

lyophilized powder

組成

Protein, ~90% biuret

標識化の程度

2-4 mol d-biotin per mol protein

テクニック

direct ELISA: 1:70,000 using human IgG

保管温度

2-8°C

詳細

Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein.
Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.

特異性

Binds IgG only from most mammalian species except cat; binds chicken IgG.

アプリケーション

Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.
Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities.

包装

Package size based on protein content

その他情報

Truncated - lacks albumin, Fab and membrane binding sites.

調製ノート

Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

保管分類コード

11 - Combustible Solids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, type N95 (US)


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

P8045-BULK-N:
P8045-PH:
P8045-BULK:
P8045-1MG-PW:
P8045-250UG:
P7693-100MG-PW:
P8045-VAR:
P8045-1MG:
P8045PROC:


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Electrochemical biosensing based on universal affinity biocomposite platforms
Zacco E, et al.
Biosensors And Bioelectronics, 21(7), 1291-1301 (2006)
Ayumi Taniguchi et al.
Journal of nutritional science and vitaminology, 53(6), 457-463 (2008-01-19)
It is well known that biotin deficiency causes morphological anomalies in hatchlings of fowl. An abundance of biotin in the yolk, therefore, is greatly required for maintaining reproductive functions. Although there is growing evidence for the molecular significance of the
Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems: Biosensors, Recognition Receptors, and Microsystem, 21(7), 360-360 (2008)
Pierre-Henri Puech et al.
PloS one, 6(7), e22344-e22344 (2011-07-30)
The rupture forces and adhesion frequencies of single recognition complexes between an affinity selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends
Alina Sultanova et al.
Microbiology spectrum, 10(3), e0236921-e0236921 (2022-05-24)
Human herpesvirus-6 (HHV-6) contains two genes (U12 and U51) that encode putative homologues of human G-protein-coupled receptors like CCR1, CCR3, and CCR5. It has been shown that these viral proteins can be expressed on the surface of epithelial and some

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