P3862

Anti-phospho-PKB (pThr³⁰⁸) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 別名: Anti-phospho-Akt
• MDL番号: MFCD02686506
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 56 kDa
• 化学種の反応性: rat, mouse
• テクニック: microarray: suitable; western blot: 1:1,000 using a whole extract of PDGF-treated mouse fibroblast NIH3T3 cells and a whole extract of H₂O₂-treated rat fibroblast Rat-1 cells
• UniProtアクセッション番号: P31750
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: phosphorylation (pThr308)
• 遺伝子情報: human ... AKT1(207) ; mouse ... Akt1(11651) ; rat ... Akt1(24185)

詳細

詳細

Protein kinase B (PKB, also known as Akt) is a serine/threonine protein kinase and a key regulator of signal transduction processes. Important processes such as cell cycle, proliferation and apoptosis are regulated by PKB in response to stimuli such as growth factors, insulin and cytokines. Three isoforms of PKB have been identified, α, β and γ. PKB α is reportedly overexpressed in breast cancer cell lines, whereas PKB β is overexpressed in ovarian and pancreatic cancers. On cell stimulation by growth factors or insulin, PKB α translocates to the cell membrane, where it undergoes full activation by phosphorylation at Thr³⁰⁸ and Ser 473, by PDK1 and PDK2. PKB α plays a crucial role in several downstream pathways involved in glycogen synthesis, protein translation and cell proliferation. PKB is also a potent inhibitor of apoptotic cell death by suppressing BAD-induced death
Anti-phospho PKB (pThr³⁰⁸) recognises PKB phosphorylated at threonine 308 (56 kDa).

免疫原

synthetic phosphopeptide corresponding to the pThr³⁰⁸ region of human PKBα (amino acids 301-315).

アプリケーション

Anti-phospho PKB (pThr³⁰⁸) may be used for detection and localization of phospho-PKB by immunoblotting. A minimum dilution of 1:1000 may be used for detection by immunoblotting in PDGF-treated mouse NIH3T3 cells and peroxide-treated Rat-1 fibroblast cell line.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable