O4264

Human Osteopontin

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

• 別名: OPN, 分泌性ホスホプロテイン1(Spp1), 初期Tリンパ球活性化タンパク質-1(ETA-1), 骨シアロタンパク質1
• MDL番号: MFCD03100698
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• product name: オステオポンチン ヒト, recombinant, expressed in NSO cells, ≥95% (SDS-PAGE), lyophilized powder, suitable for cell culture
• 由来生物: human
• 品質水準: 100
• リコンビナント: expressed in NSO cells
• 無菌性: sterile
• アッセイ: ≥95% (SDS-PAGE)
• 形状: lyophilized powder
• 分子量: 60-65 kDa by SDS-PAGE (reducing)
• 包装: pkg of 50 μg
• テクニック: cell culture | mammalian: suitable
• 不純物: endotoxin, tested
• UniProtアクセッション番号: P10451
• 輸送温度: ambient
• 保管温度: −20°C
• 遺伝子情報: human ... SPP1(6696)

詳細

詳細

Secreted phosphoprotein 1 (SPP1) or osteopontin is a secreted cytokine. This phosphorylated acidic glycoprotein is expressed in various cells and tissues. It is synthesized by osteoblasts. The gene encoding SPP1 is localized on human chromosome 4q22.1.

アプリケーション

Anti-Osteopontin antibody produced in rabbit has been used for ELISA.

生物化学的／生理学的作用

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity. Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization. It is a marker of many cancers.

物理的形状

凍結乾燥品 (PBS溶液, 2.5mg BSA含有, 0.2μmフィルタ-濾過)。

アナリシスノート

293細胞に対する接着作用により生物活性を測定しています。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)