N9414

抗NUCB2 (N-末端) ウサギ宿主抗体

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: 抗Calnuc抗体, 抗NEFA抗体, 抗Nef抗体, 抗p54抗体, 抗ヌクレオバインディン2抗体, 抗ネスファチン抗体
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~54 kDa
• 化学種の反応性: rat, mouse
• 濃度: ~1.5 mg/mL
• テクニック: indirect immunofluorescence: 1-2 μg/mL using Rat2 cells; western blot: 1.5-3.0 μg/mL using Rat2 cell lysates; western blot: 2-4 μg/mL using mouse testis extracts (S1 fraction)
• UniProtアクセッション番号: P81117
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: rat ... Nucb2(59295)

詳細

詳細

NUCB2 (N-terminal) (nucleobindin 2, also known as NEFA, p54) belongs to the nucleobindin family of EF-hand Ca²⁺-binding proteins.

Nucleobindin 2 (NUCB2), contain multiple functional domains, including a Leu/Ile-rich region, a putative nuclear localization signal, a DNA binding domain, two Ca²⁺ -binding EF-hand motifs, and a leucine zipper region. NUCB2 is widely distributed and is localized to the Golgi apparatus. Human NUCB2 has high homology (62% sequence identity) with human NUCB1, an additional member of the nucleobindin family, although they are encoded by two separate genes.

アプリケーション

Anti-NUCB2 (N-terminal) antibody produced in rabbit has been used in:

• immunofluorescence
• western blotting
• immunohistochemistry

生物化学的／生理学的作用

Nucleobindin 2 (NUCB2) is implicated in various functions, including Ca²⁺ homeostasis, hypothalamic regulation of feeding, and tumor necrosis factor (TNF)-receptor-1 (TNFR1) shedding. NUCB2 has been shown to interact with both necdin and endoplasmic reticulum aminopeptidase 1 (ARTS-1), both through the EF-hand motif. NUCB2-ARTS-1 complex is required for the proteolytic cleavage and release of TNFR1 to the extracellular compartment. NUCB2 has been recently identified as a novel satiety molecule in the hypothalamus, associated with melanocortin signaling in the central nervous system. NUCB2 and its N-terminal 82-amino acid cleavage fragment, termed nesfatin-1, have been shown to induce suppression of food-intake in rats.

物理的形状

0.01M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable