N2783

NOシンターゼ, 誘導型 マウス由来

recombinant, expressed in E. coli, buffered aqueous solution

• 別名: NOS II, iNOS, macNOS, 誘導型NOシンターゼ
• Enzyme Commission number: 1.14.13.39 (BRENDA, IUBMB)
• MDL番号: MFCD00803363
• UNSPSCコード: 12352303
• NACRES: NA.32

特性

• リコンビナント: expressed in E. coli
• 品質水準: 200
• フォーム: buffered aqueous solution
• 比活性: ≥4.0 units/mg protein
• 分子量: 130 kDa (homodimer); 130 kDa (subunit, homodimer)
• UniProtアクセッション番号: P29477
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: mouse ... Nos2(18126)

詳細

詳細

Inducible nitric oxide synthase (iNOS), also known as inflammatory nitric oxide synthase, is a calcium independent isoenzyme, involved in synthesis of nitric oxide (NO). It is a soluble enzyme encoded by the gene mapped to mouse chromosome 11. iNOS is active in dimeric form and its activity is induced by cytokines and various other stimuli. iNOS is expressed in various inflammatory conditions.

アプリケーション

Nitric Oxide Synthase, Inducible from mouse has been used in immunohistochemical studies. It is also used to evaluate the therapeutic efficacy of inducible nitric oxide synthase (NOS) on reperfusion-induced microcirculatory alterations and hemodynamic adverse effects in the microvasculature of skeletal muscle.

生物化学的／生理学的作用

NOSは、L-アルギニンからの一酸化窒素の生合成に関与しています。iNOSは、カルシウム/カルモジュリンに依存しません。L-アルギニンに対するK_m値は16 μMです。

Tumor-derived inducible nitric oxide synthase (iNOS) plays a vital role in stimulating tumor growth and vessel maturation. Therefore, it is considered to be a potential therapeutic target for anti-vascular cancer therapies. Unchecked activity of iNOS leads to overproduction of nitric oxide (NO), which is toxic for living cells. iNOS activity can be controlled at both transcription and translational level by regulating protein stability, dimerization, phosphorylation, cofactor binding and availability of oxygen and L-arginine as substrates. iNOS plays a vital role in excisional wound repair and exhibits gene therapy strategy to advance wound healing process in iNOS-deficient conditions such as diabetes and steroid treatment.

単位の定義

酵素1ユニットは、50 mM HEPES(pH 7.4、4.5 μMオキシヘモグロビン、0.15 mM NADPH、1 mMアルギニン、1 mM酢酸マグネシウム、18 μMテトラヒドロビオプテリン、180 μM DTT含有)中、37°Cで1分間に1 nmolの一酸化窒素を生成する量です。

物理的形状

50 mM HEPES溶液(pH 7.4, 10%グリセロール 8 μMテトラヒドロビオプテリン含有)

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable