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Merck
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主要文書

安全性情報

MBD0013

Sigma-Aldrich

Microbial DNA standard from Escherichia coli

Suitable for PCR, sequencing and NGS, 10 ng/μL

別名:

E.Coli

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About This Item

UNSPSCコード:
41105500
NACRES:
NA.51

品質水準

形状

liquid

濃度

10 ng/μL

テクニック

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

適合性

suitable for restriction endonuclease digests, PCR amplification, Southern blots, and sequencing reactions

輸送温度

ambient

保管温度

−20°C

詳細

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Escherichia coli genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs. Escherichia coli is a gram negative, facultative aerobic, rod-shaped coliform bacterium. E. coli colonizes the infant gut within hours of birth and establishes itself as the most abundant facultative anaerobe of the human intestinal microflora for the remainder of life, equipped with the abilities to grow in the ever-changing environment in the gut and cope with the mammalian host interaction. Nevertheless, E. coli can survive in many different ecological habitats, including abiotic environments, and is considered a highly versatile species. Known habitats of E. coli include soil, water, sediment, and food. Some strains of E. coli have evolved and adapted to a pathogenic lifestyle and can cause different disease pathologies.


Read here how to use our standards to ensure data integrity for your microbiome research.

アプリケーション

The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

特徴および利点

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

物理的形状

Liquid - The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0

その他情報

Strain: MG1655

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

MBD0013-0.3UG-PW:
MBD0013-0.3UG:
MBD0013-VAR:
MBD0013-BULK:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Order and disorder during Escherichia coli divergence.
Heather Hendrickson
PLoS genetics, 5(1), e1000335-e1000335 (2009-01-24)
Lisa C Crossman et al.
Journal of bacteriology, 192(21), 5822-5831 (2010-08-31)
In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible
James B Kaper et al.
Nature reviews. Microbiology, 2(2), 123-140 (2004-03-26)
Few microorganisms are as versatile as Escherichia coli. An important member of the normal intestinal microflora of humans and other mammals, E. coli has also been widely exploited as a cloning host in recombinant DNA technology. But E. coli is
J Paul Brooks et al.
BMC microbiology, 15, 66-66 (2015-04-17)
Characterizing microbial communities via next-generation sequencing is subject to a number of pitfalls involving sample processing. The observed community composition can be a severe distortion of the quantities of bacteria actually present in the microbiome, hampering analysis and threatening the

資料

An overview of human microbiome research, workflow challenges, sequencing, library production, data analysis, and available microbiome reagents to support your research.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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