MAK538

Ammonia Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• UNSPSCコード: 12161503
• NACRES: NA.84

特性

• 品質水準: 100
• 検出方法: colorimetric; fluorometric
• 関連疾患: gastrointestinal diseases
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Ammonia (NH3) or its ion form ammonium (NH4 +) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase).

特徴および利点

• Linear detection range: 24 to 1000 µM

適合性

The kit is suitable for ammonia determination in biological samples (e.g. serum, plasma, urine, saliva, cell culture, etc).

原理

The Ammonia Assay Kit is designed to directly measure NH₃ and NH4 +. In this assay, NADH is converted to NAD⁺ in the presence of NH₃, ketoglutarate and glutamate dehydrogenase. The decrease in optical density at 340 nm or fluorescence intensity at λEx = 360 nm/λEm = 450 nm is directly proportionate to the NH3 concentration in the sample.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3