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使用法
sufficient for 40 extractions
環境により配慮した代替製品の特徴
Waste Prevention
Learn more about the Principles of Green Chemistry.
sustainability
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アプリケーション
cosmetics
food and beverages
sample preservation
環境により配慮した代替製品カテゴリ
保管温度
room temp
関連するカテゴリー
詳細
Cholesterol is a structural component of cell membranes and a precursor to hormones. The Cholesterol Extraction Kit enables the extraction of sterols including cholesterol from biological samples in two simple steps. The extracted sterols can then be saponified, derivatized, and quantified using gas chromatography (GC) with flame-ionization detection (FID) or gas chromatography/mass spectrometry (GC/MS).
適合性
This kit is suitable for the extraction of cholesterol and cholesterol-like compounds (sterols) from whole blood, serum, biological tissue and food samples.
原理
Sterols including cholesterol are typically extracted using a dual solvent partition system containing a lipophilic solvent and an aqueous solvent. Sterols are retained in the lower chloroform phase, whereas, aqueous-soluble compounds are retained in the methanol-water layer. The sample is then centrifuged and the bottom chloroform layer is transferred with a pipette to another test tube. An aliquot of the transferred layer is then saponified with methanolic NaOH or KOH, and then derivatized with trimethylsilyl chloride (chlorotrimethylsilane). The formed cholesterol-TMS ester is reconstituted with hexane and injected directly into a GC-FID or GC/MS system for quantification.
The Cholesterol Extraction Kit shortens the extraction process by eliminating the need to prepare solvents and standards, centrifugation, and pipetting. Once the sample is homogenized and dissolved in the Extraction Solvent containing the internal standard, it is inverted twice and poured into the syringe containing a filter, which preferentially elutes the chloroform layer containing sterols. A portion of the total sterol extract can be saponified and derivatized for GC-FID or GC/MS analysis as described in the procedure.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods.Find details here.
The Cholesterol Extraction Kit shortens the extraction process by eliminating the need to prepare solvents and standards, centrifugation, and pipetting. Once the sample is homogenized and dissolved in the Extraction Solvent containing the internal standard, it is inverted twice and poured into the syringe containing a filter, which preferentially elutes the chloroform layer containing sterols. A portion of the total sterol extract can be saponified and derivatized for GC-FID or GC/MS analysis as described in the procedure.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods.Find details here.
アナリシスノート
Click on the following link for a demo: Easy to Use High-Throughput Kits for Cholesterol and Lipid Extractions
その他情報
Easy to Use High-Throughput Kits for Cholesterol and Lipid Extractions. Watch the Video to learn more and to see how the technology works.
シグナルワード
Danger
危険有害性の分類
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 1 - STOT SE 3
ターゲットの組織
Central nervous system, Eyes, Liver,Kidney
保管分類コード
3 - Flammable liquids
引火点(°F)
49.5 °F - closed cup
引火点(℃)
9.7 °C - closed cup
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
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PRTR
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消防法
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労働安全衛生法名称等を表示すべき危険物及び有害物
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労働安全衛生法名称等を通知すべき危険物及び有害物
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カルタヘナ法
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Jan Code
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試験成績書（COA）
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