MAK162

Colorimetric Maleimide Assay Kit

sufficient for 100 colorimetric tests

• UNSPSCコード: 12161503
• NACRES: NA.84

特性

• 使用法: sufficient for 100 colorimetric tests
• 検出方法: colorimetric
• 関連疾患: cancer
• 保管温度: −20°C

詳細

詳細

Maleimide is an unsaturated imide used in protein conjugation reactions. The direct measurement of maleimide moieties following conjugation reactions by spectrophotometric analysis at 302 nm is insensitive due to the small extinction coefficient (620M-1 cm-1) and complicated by protein absorbance at this wavelength.

適合性

Suitable for quantifying maleimide groups

原理

The colorimetric maleimide quantiation kit provides a simple and direct procedure for measuring maleimide groups. The sample is first reacted with a known amount of excess thiol and then the remaining unreacted thiol is assayed using 4,4′-DTDP (4,4′-Dithiodipyridine). The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H302,H317,H319,H335
• 注意書き: P261 - P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Sens. 1 - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): 188.6 °F - closed cup
• 引火点(℃): 87 °C - closed cup