M8320

抗MLH1 (N末端) 抗体 ウサギ宿主抗体

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: 抗COCA2抗体, 抗FCC2抗体, 抗HNPCC抗体, 抗MGC5172抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 80-85 kDa
• 化学種の反応性: rat, human, mouse
• 包装: antibody small pack of 25 μL
• 濃度: ~1 mg/mL
• テクニック: immunocytochemistry: 2.5-5 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton; immunoprecipitation (IP): 5-10 μg using Jurkat cell lysates; western blot: 0.5-1 μg/mL using Jurkat cell lysates
• UniProtアクセッション番号: P40692
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MLH1(4292) ; mouse ... Mlh1(17350) ; rat ... Mlh1(81685)

詳細

詳細

MutL homolog 1 (MLH1) is a nucleoprotein and a major component of mismatch repair system. The MLH1 gene is localized on chromosome 3p21 and is made up of 19 exons. The protein has a molecular weight of 80kDa.

MutL homolog 1 (MLH1) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors and signal transducers, named BRCA1-associated genome surveillance complex (BASC).

免疫原

ヒトMLH1のアミノ酸 60~75に相当する合成ペプチド (N末端システイン付加) のKLH結合体。相当するペプチド配列はヒト、ラット、マウスで保存されています。

アプリケーション

• Anti-MLH1 (N-terminal) antibody produced in rabbit has been used in:
• immunoblotting
• immunoprecipitation
• immunocytochemistry

生物化学的／生理学的作用

MutL homolog 1 (MLH1) has been shown to be involved in stimulating carcinogenesis in the colon.

MutL protein (MLH), a homolog of the E. coli MutL gene, is involved in DNA mismatch repair. Nonpolyposis colorectal cancer-2 is caused by a hereditary mutation in the MLH1 gene. This cancer can also be a result of hypermethylation of one MLH1 allele in somatic cells (a germline epimutation).

ターゲットの説明

MLH1 (N-terminal) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors, and signal transducers, named BASC (BRCA1-associated genome surveillance complex).

物理的形状

0.01 M PBS溶液 (pH 7.4, 15 mM アジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)