M3567

抗ミオシンI β(核)抗体 ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: 抗Myh 2抗体, 抗Myo1c抗体, 抗Myr 2抗体
• MDL番号: MFCD05665241
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~120 kDa
• 化学種の反応性: human, mouse, canine, rat
• テクニック: indirect immunofluorescence: 1:50 using mouse NIH3T3 cells; microarray: suitable; western blot: 1:1,000 using extract of human HeLa cells nuclei and dog MDCK cells
• UniProtアクセッション番号: O00159
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MYO1C(4641) ; mouse ... Myo1c(17913) ; rat ... Myo1c(65261)

詳細

詳細

Myosins belong to a superfamily of actin-based motor proteins comprising to date at least 15 classes. Myosin I (also designated myosin-1c, myo1c, M1C, myr 2 and myh 2) is a widely distributed single-headed myosin composed of an N-terminal motor domain, a calmodulin-light chain binding neck region, and a short C-terminal domain. In mammalian cells, myosin I is usually found in the cytoplasm and is especially enriched in perinuclear regions and dynamic cell margins. Myosin Iβ is found in a variety of cells such as kidney tubular cells and inner-ear hair cells.

特異性

核型ヒトミオシンIβのH鎖を認識します。サンプルによっては別のバンドが検出される場合もあります。

免疫原

マウスミオシンIβ(核型)のH鎖のアミノ酸残基1-16に相当する合成ペプチド。この配列はミオシンIの他のアイソフォ-ムをはじめ、その他既知のミオシン分子にも存在しません。

アプリケーション

Anti-Myosin Iβ (Nuclear) antibody produced in rabbit has been used in:

• western blot
• confocal immunofluorescence
• immunogold labeling
• immunofluorescence
• co-immunoprecipitation experiments
• immunostaining

生物化学的／生理学的作用

Myosin I is involved in cell motility, microvilli anchorage, vesicular and organelle transport and signal transduction. Myosin I associate with lipid membranes through its tail domain while its motor domain interacts with actin filaments. Myosin Iβ (nuclear) co-localizes with RNA polymerases I and II and possibly acts as a molecular motor to power transcription.

物理的形状

0.01M PBS溶液 (pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable