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由来生物
goat
品質水準
結合体
unconjugated
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
フォーム
lyophilized powder
化学種の反応性
rat
テクニック
capture ELISA: 0.2-0.8 μg/mL
flow cytometry: 2.5 μg using 106 rat cortical stem cells
western blot: 0.1 μg/mL
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... JAG1(182)
rat ... Jag1(29146)
詳細
Notch signaling is a well conserved among the metazoans and is a regulator of growth, differentiation, and patterning processes. One of the ligands of Notch family receptors is Jagged. Mammalian Jagged family comprises of Jagged-1 and Jagged-2. Jagged proteins are expressed during the critical stages of embryonic and postnatal development. The Notch-Jagged signaling regulates the proliferation of endothelial cells, apoptosis, differentiation of hematopoietic progenitors, skeletal development and formation and differentiation of blood vessels. Notch-jagged pathway components play important role in the pathology of diseases like Alagille syndrome, CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy) and osteosarcoma. Anti-Jagged 1 antibody specifically recognizes rat Jagged-1.
免疫原
ラットJagged1細胞外ドメインをNSO細胞で発現させたリコンビナントタンパク質。
アプリケーション
Anti-Jagged 1 antibody may be used for ELISA at a working antibody concentration of 0.5-1.0 μg/ml. For immunoblotting, antibody concentration of 0.1-0.2 μg/ml is recommended. For flow cytometry, a concentration of 20 μg/ml (10 μL/105 cells to 106 cells) may be used. The antibody is suitable for neutralization assays (ND50 is ~2-10 μg/ml).
ターゲットの説明
Jagged-1はNotchのリガンドです。Notchシグナル伝達系は、造血組織を含む多くの組織において、増殖・分化・アポト-シスを調節します。Notchは膜貫通型受容体ファミリ-に属し、隣接細胞に発現するリガンド (Jagged-1, Jagged-2, Delta-1)により活性化されます。 造血幹細胞および初期前駆細胞はNotch-1, Notch-2を、間質系の初代培養細胞はJagged-1を発現します。これはJagged-1が造血微小環境における前駆細胞の成育に関与することを示しています。Jagged-1を高発現させた細胞は、造血系前駆細胞コロニ-形成を4倍に増加させました。しかし、増殖および成熟を刺激するIL-3, IL-6, SCF, G-CSFのような他のサイトカインの非存在下では、Jagged-1はCD34+陽性細胞における骨髄細胞系コロニ-形成を減少させました。これによりJagged-1はサイトカインシグナルを調節し未成熟CD34+細胞の維持に寄与すると示唆されました。
物理的形状
凍結乾燥品 (PBS溶液, 0.2μmフィルタ-濾過)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
J4127-.1MG-PW:
J4127-BULK:
J4127-.1MG:
J4127-VAR:
Chandramouli R Chillakuri et al.
Seminars in cell & developmental biology, 23(4), 421-428 (2012-02-14)
The Notch receptor is part of a core signalling pathway which is highly conserved in all metazoan species. It is required for various cell fate decisions at multiple stages of development and in the adult organism, with dysregulation of the
L Walker et al.
Stem cells (Dayton, Ohio), 17(3), 162-171 (1999-05-26)
The cell surface receptor Notch1 is expressed on CD34+ hematopoietic precursors, whereas one of its ligands, Jagged1, is expressed on bone marrow stromal cells. To examine the role of Notch signaling in early hematopoiesis, human CD34+ cells were cultured in
L Miele et al.
Journal of cellular physiology, 181(3), 393-409 (1999-10-21)
Notch-ligand interactions are a highly conserved mechanism that regulates cell fate decisions. Over the past few years, numerous observations have shown that this mechanism operates to regulate cell differentiation in an enormous variety of developmental and cell maturation processes. Recent
Stefano Zanotti et al.
Molecular and cellular biology, 30(4), 886-896 (2009-12-10)
Notch receptors are transmembrane receptors that regulate cell fate decisions. There are four Notch receptors in mammals. Upon binding to members of the Delta and Jagged family of transmembrane proteins, Notch is cleaved and the Notch intracellular domain (NICD) is
Cristina Roca et al.
Genes & development, 21(20), 2511-2524 (2007-10-17)
The Notch pathway is a versatile regulator of cell fate specification, growth, differentiation, and patterning processes in metazoan organisms. In the vertebrate cardiovascular system, multiple Notch family receptors and several of their Jagged and Delta-like ligands are expressed during critical
資料
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
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