I2534

モノクローナル抗インポーチンβ抗体 マウス宿主抗体

clone 31H4, purified from hybridoma cell culture

• 別名: 抗Imp β抗体, 抗p97抗体, 抗カリオフェリンβ1抗体
• MDL番号: MFCD07785407
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: 31H4, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~97 kDa
• 交差性: rat, human, mouse, bovine
• テクニック: immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 0.5-1 μg/mL using HeLa total cell extract
• アイソタイプ: IgG1
• UniProtアクセッション番号: Q14974
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... KPNB1(3837) ; mouse ... Kpnb1(16211) ; rat ... Kpnb1(24917)

詳細

詳細

Importin β comprises 19 tandemly repeated Huntingtin, elongation factor 3 (EF3), protein phosphatase 2A (PP2A), and the yeast kinase serine/threonine-protein kinase-TOR1 (HEAT) motifs and wraps around the importin-β (IBB). It also consists of an N-terminal RAN guanosine triphosphate (GTP) binding domain, a middle zone interacting with the nuclear pore, and an importin α binding domain at the C-terminus. The importin β gene is mapped to human chromosome location 17q21.32.

Monoclonal Anti-Importin b (mouse IgG1 isotype) is derived from the 31H4 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with importin. Importin β (Imp β), also referred to as karyopherin β1, is an import receptor that belongs to the family of nuclear transport receptors.

特異性

Monoclonal Anti-Importin β recognizes human, bovine, rat, and mouse importin β.

免疫原

インポ-チンβ。

アプリケーション

Anti-Importin β antibody,Mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunocytochemistry
• immunofluorescent staining
• coimmunoprecipitaion
• western blotting
• immunofluorescence
• immunoprecipitation

生物化学的／生理学的作用

Importin β (Impβ) forms a complex with importin α, which, in turn, binds the cargo protein via its NLS (nuclear localization signals). The cargo protein is transported by this ternary complex to the cytoplasmic face of the nuclear pore complex (NPC) via a direct interaction with specific nucleoporins. When the complex reaches the nuclear site of the nuclear pore complex (NPC), Ran-GTP binds to Impβ to form an Impβ/Ran-GTP complex and releases Impα and the cargo protein. The Impβ/Ran-GTP complex is then transported back to the cytoplasm, where the complex dissociates upon hydrolysis of GTP to GDP. Importin β is then ready to participate in new nuclear import cycle.

Importin β regulates cellular functions, such as mitotic spindle assembly and centrosome dynamics. It is implicated in the insertion of integral membrane proteins into the inner nuclear membrane. Importin β also functions as a chaperone for highly basic proteins in the cytoplasm.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable