HPA015234

Anti-PREX2 antibody produced in rabbit

Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

• 別名: Anti-DEP.2, Anti-DEPDC2, Anti-FLJ12987, Anti-P-REX2, Anti-PPP1R129
• UNSPSCコード: 12352203
• Human Protein Atlas Number: HPA015234
• NACRES: NA.43
• 結合体: unconjugated
• application: IHC
• クローン: polyclonal
• 化学種の反応性: human
• citations: 8
• テクニック: immunohistochemistry: 1:1000- 1:2500

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 製品種目: Prestige Antibodies^® Powered by Atlas Antibodies
• フォーム: buffered aqueous glycerol solution
• 化学種の反応性: human
• 強化検証: orthogonal RNAseq; Learn more about Antibody Enhanced Validation
• テクニック: immunohistochemistry: 1:1000- 1:2500
• 免疫原配列: GLGFQIRGFGPSVVHAVGRGTVAAAAGLHPGQCIIKVNGINVSKETHASVIAHVTACRKYRRPTKQDSIQWVYNSIESAQEDLQKSHSKPPGDEAGDAFDCKVEEVIDKFNTMAIIDGKKEHVSLTVDNVHLEYGVVYEYDSTAGIKCNV
• UniProtアクセッション番号: Q70Z35
• アプリケーション: research pathology
• 輸送温度: wet ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PREX2(80243)

詳細

詳細

PREX2 (phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2) is a homolog of PREX1, and belongs to the Dbl (Diffuse B-cell lymphoma) family guanine nucleotide exchange factors (GEFs) family. It contains a PH (pleckstrin homology) domain, which is essential for substrate recognition. It also contains DH (Dbl-homology) domain and PDZ domains. This protein has a molecular weight of 183kDa.

免疫原

ホスファチジルイノシト-ル3,4,5-三リン酸依存性Rac交換体2タンパク質のPrEST(protein epitope signature tag)抗原リコンビナントタンパク質。

アプリケーション

Prestige抗体^®は、Human Proteome Resource(HPR)プロジェクト(www.proteinatlas.org))によって開発・実証されたAtlas抗体です。抗体はすべて、数百の正常組織・疾病組織に対する免疫組織染色試験を行っています。これらの染色画像はHuman Protein Atlas(HPA)サイトで[Image Gallery]リンクをクリックするとご覧いただけます。さらに、ほとんどのPrestige抗体はプロテインアレイおよびウェスタンブロッティングの試験を行っています。試験のプロトコールおよびPrestige抗体、HPAに関する情報はsigma.com/prestigeをご覧ください。

生物化学的／生理学的作用

PREX2 (phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2) interacts with and suppresses PTEN (phosphatase and tensin homolog) protein in breast cancer. Mutations in this gene are linked to human melanomas, and abnormal expression of this gene leads to tumorigenesis in immortal melanocytes. Mutations in this gene are also associated with lung, colon and pancreatic cancer. Thus, mutations are thought to turn PREX2 in an oncogene. It recognizes and interacts with Rac-1 protein, RhoA and Cdc42, and activates them. This, in turn, is activated by PI-3K (Phosphoinositide 3-kinase). It is induced by CXCL9 (Chemokine (C-X-C Motif) Ligand 9), which results in enhanced invasiveness of hepatocellular carcinoma. PREX2 is also up-regulated in gastric cancer (GC), and this expression is suppressed by miR-338-3p, which acts as a tumor suppressor in GC.

特徴および利点

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

関連事項

Corresponding Antigen APREST72469

物理的形状

PBS溶液(pH 7.2, 40%グリセロールおよび0.02%アジ化ナトリウム含有)。

法的情報

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)