H7165

Anti-HtrA2 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: Anti-High Temperature Requirement protein A2, Anti-HtrA serine peptidase-2, Anti-OMI, Anti-PARK13, Anti-PRSS25, Anti-Protease, Serine, 25
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~36 kDa
• 交差性: rat, human
• 包装: antibody small pack of 25 μL
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: ~1.5 mg/mL
• テクニック: western blot: 2-4 μg/mL using rat liver microsomal fraction
• UniProtアクセッション番号: O43464
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... HTRA2(27429) ; mouse ... Htra2(64704) ; rat ... Htra2(297376)

詳細

詳細

High temperature requirement protein A2 (HtrA2) or serine peptidase 2 belongs to the HtrA family of serine proteases. It is a mitochondrial serine protease, that comprises of an N-terminal mitochondrial localization sequence (MLS), an inhibitor of apoptosis protein (IAP) binding domain (IBM) and a single C-terminal PDZ domain. It also has a transmembrane and a serine protease domain. HTRA2 gene is mapped to human chromosome 2p13.1.

特異性

Anti-HtrA2 (C-terminal) specifically recognizes human and rat HtrA2.

アプリケーション

Anti-HtrA2 (C-terminal) antibody produced in rabbit may been used in immunoblotting.

生物化学的／生理学的作用

High temperature requirement protein A2 (HtrA2) exhibits endoproteolytic activity by cleaving misfolded proteins and other cellular proteins. It is essential for mitochondrial homeostasis and regulates apoptosis through multiple pathways, including caspase-dependent and caspase-independent cell death. Mature HtrA2, via its IAP binding domain favors the neutralizing inhibitor of apoptosis proteins (IAPs) by promoting the cytochrome c-dependent caspase activation. Mutations that inactivate HtrA2 gene are associated with neurodegenerative diseases such as Parkinson′s disease and Alzheimer′s disease. Mutation in the HtrA2 gene is also implicated in the early-onset mitochondrial syndromes including the 3-methylglutaconic aciduria. HtrA2 variants are responsible infantile neurodegeneration, mitochondrial dysfunction and promote apoptosis.

物理的形状

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)