H3159

抗ヒストンデアセチラーゼ2 (HDAC2) ウサギ宿主抗体

IgG fraction of antiserum, buffered aqueous solution

• 別名: Anti-HD2, Anti-KDAC2, Anti-RPD3, Anti-YAF1
• MDL番号: MFCD02096544
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 55 kDa
• 化学種の反応性: mouse, human
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested, human lymph node sections; immunoprecipitation (IP): 50 μg using whole lysate of NIH3T3 cells; microarray: suitable; western blot: 1:20,000 using nuclear extract from human HeLa cells
• UniProtアクセッション番号: Q92769
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... HDAC2(3066) ; mouse ... Hdac2(15182)

詳細

詳細

Histone Deacetylase (HDACs) have been described as six or seven different groups in mammals. HDAC1, HDAC2 and HDAC3 are like yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are like yeast Hda1 (Histone Deacetylase 1) protein.

特異性

Anti-Histone Deacetylase 2 specifically recognizes histone deacetylase 2 by immunoblotting and immunoprecipitation (55 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC2 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC2 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin embedding. The antibody reacts with HDAC2 of human, rat and mouse origin.

免疫原

ヒトHDAC2のアミノ酸配列471~488に相当する合成ペプチド。この配列はマウスとニワトリで高度に保存されています。

アプリケーション

Anti-Histone Deacetylase 2 (HDAC2) antibody has been used:

• in immunoblotting and dot blot
• in immunoprecipitation
• in immunohistochemistry
• in enzyme linked immunosorbent assay (ELISA)
• in western blotting
• in chromatin immunoprecipitation(ChiP)

生物化学的／生理学的作用

Histone acetylation is a dynamic process whose levels are determined by the net activities of Histone acetyl transferase (HATs) and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosome condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.

物理的形状

0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable