おすすめの製品
メーカー／製品名
Cytiva RPN226
包装
pkg of 96 wells
保管温度
2-8°C
関連するカテゴリー
詳細
EIAs and ELISAs are convenient, high-sensitivity, peroxidase-based technologies that enable the amount of protein present in a sample to be measured in a single day. Novel lysis reagents have been developed (Lysis Reagents 1 and 2) to facilitate simple and rapid extraction of cellular molecules.
The Biotrak™ cGMP competitive enzymeimmunoassay system is specifically designed for research purposes. The kit includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of cGMP from cell cultures. These components avoid the requirement for traditional, time-consuming extraction procedures and obviate the need for removal of extraction reagents prior to measurement. It combines the use of a peroxidase-labeled cGMP conjugate, a specific antiserum which can be immobilized on to precoated microplates, and a one-pot stabilized substrate solution.
Lysis reagent 1 hydrolyzes cell membranes to release intra-cellular cAMP. Lysis Reagent 2 sequesters the key component in Lysis Reagent 1 and ensures cAMP is free for subsequent analysis. The detergent/sequestrant complex does not interfere with antigen: antibody binding. Lysis Reagent 1 is simply added to cultured cells, followed by a 5 to 10 min incubation before assay. The antiserum is reconstituted with Lysis Reagent 2. The assay is based on competition between unlabeled cAMP and a fixed quantity of peroxidase-labeled cAMP, for a limited number of binding sites on a cAMP specific antibody.
The Biotrak™ cGMP competitive enzymeimmunoassay system is specifically designed for research purposes. The kit includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of cGMP from cell cultures. These components avoid the requirement for traditional, time-consuming extraction procedures and obviate the need for removal of extraction reagents prior to measurement. It combines the use of a peroxidase-labeled cGMP conjugate, a specific antiserum which can be immobilized on to precoated microplates, and a one-pot stabilized substrate solution.
Lysis reagent 1 hydrolyzes cell membranes to release intra-cellular cAMP. Lysis Reagent 2 sequesters the key component in Lysis Reagent 1 and ensures cAMP is free for subsequent analysis. The detergent/sequestrant complex does not interfere with antigen: antibody binding. Lysis Reagent 1 is simply added to cultured cells, followed by a 5 to 10 min incubation before assay. The antiserum is reconstituted with Lysis Reagent 2. The assay is based on competition between unlabeled cAMP and a fixed quantity of peroxidase-labeled cAMP, for a limited number of binding sites on a cAMP specific antibody.
アプリケーション
cGMP, Biotrak™ EIA System has been used for measuring total cellular cyclic GMP (cGMP) or cyclic AMP (cAMP) levels.
特徴および利点
- Elimination of inconvenient, time-consuming extraction procedures.
- Flexible method-choice of assay protocols.
- Dual range 2–512 fmol/well (acetylation protocol), dual range 50–12 800 fmol/well (non-acetylation protocol).
- Rapid assay protocol.
- Nonradioactive.
- Specific for cGMP
- Precise and accurate measurement.
- Ready-to-use substrate.
- Colour coded reagents
保管および安定性
Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
Biotrak is a trademark of Cytiva
シグナルワード
Danger
危険有害性情報
保管分類コード
3 - Flammable liquids
最新バージョンのいずれかを選択してください：
Ca2+ -permeable AMPA (-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid) Receptors and Dopamine D1 Receptors Regulate GluA1 Trafficking in Striatal Neurons
David S T and Edward B Z
The Journal of Biological Chemistry, 288(9) (2013)
YC-1 potentiates the nitric oxide/cyclic GMP pathway in corpus cavernosum and facilitates penile erection in rats.
Gin CH, et al.
European Journal of Pharmacology, 458(1-2), 183-189 (2003)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）