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GE29-0915-96

Sigma-Aldrich

Superose® 6 Increase 10/300 GL

Cytiva 29-0915-96, 10/300 GL

別名:

Superose® 6 Increase

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About This Item

UNSPSCコード:
41115700
NACRES:
SB.52

物質

glass column
polyethylene filter

製品種目

Superose®

分子量

5000-5,000,000 Da (globular proteins)

包装

package of 1 ea

メーカー／製品名

Cytiva 29-0915-96

パラメーター

<0.35 mL/min flow rate
<0.75 mL/min flow rate (20% ethanol at room temp)
<0.75 mL/min flow rate
<1.50 mL/min flow rate (water at room temp)
25-500 μL sample volume
3 Mpa

ベッド容積

24 mL

Matrix

cross-linked agarose
polymeric

マトリックス活性基

agarose phase

cleaning

1-14

動作範囲

3-12

分離法

size exclusion (SEC)

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詳細

Superose® 6 Increase 10/300 GL is a versatile, prepacked column for high-resolution size exclusion chromatography in small-scale (< 500 μl sample volume) preparative purification, as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000.

特徴および利点

  • Increased resolution compared with Superose 6, for higher purity and improved analysis results.
  • Reduced runtime compared with Superose 6, for rapid results.
  • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
  • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

適合性

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)
Superose® 6 Increase 10/300 GL column is suitable for small-scale (25 – 500 μl sample volume) preparative purification for obtaining milligram amounts of highly size-homogenous target proteins at the polishing stage of a purification procedure. It is also well-suited for the characterization of large protein complexes and for the study of protein-protein interactions.

保管および安定性

Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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法的情報

Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

保管分類コード

3 - Flammable liquids

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試験成績書（COA）

Lot/Batch Number

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文書ライブラリにアクセスする

Anja Fux et al.
Cell chemical biology, 26(1), 48-59 (2018-11-13)
Detection of dynamic protein-protein interactions within complexes and networks remains a challenging task. Here, we show by the example of the proteolytic ClpXP complex the utility of combined chemical cross-linking and mass spectrometry (XL-MS) to map interactions within ClpP and

資料

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

プロトコル

Performing a Separation with Superose

Superose from GE Healthcare are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

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