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Merck

GE29-0513-24

HiTrap® SP HP

Cytiva 29-0513-24, pack of 1 mL

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About This Item

UNSPSCコード:
23151816
NACRES:
NA.56

ligand

sulphopropyl

詳細

Ion Exchanger Type (value)

包装

pack of 1 mL

メーカー/製品名

Cytiva 29-0513-24

利用可能性

not available in North America

パラメーター

42 psi

ベッドサイズ

7 mm × 25 mm

ベッド容積

1 mL

カラム内径

7 mm

Matrix

6% cross-linked agarose

粒径

24-44 μm

平均部品サイズ

34 μm

cleaning

3-14

動作範囲

4-13

適合性

suitable for bioprocess medium

詳細

HiTrap SP HP are prepacked, ready-to-use SP Sepharose High Performance strong cation exchange columns for high-resolution, small-scale protein purification.

アプリケーション

The small particle size (34 μm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose High Performance is a strong cation exchange medium and Q Sepharose High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.

特徴および利点

  • 34 μm bead size for high-performance, high-resolution purifications.
  • Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
  • Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
  • Strong sulfopropyl (SP) cation exchanger.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

HiTrap is a registered trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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試験成績書(COA)

Lot/Batch Number

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資料

This page shows how to perform a purification of His-tagged membrane proteins.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

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プロトコル

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

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