GE29-0491-04

HiTrap^® MabSelect SuRe^™

Cytiva 29-0491-04, pack of 1 mL

• UNSPSCコード: 23151816
• NACRES: NA.56

特性

• ligand: alkali-stabilized protein A-derived (E. coli)
• 包装: pack of 1 mL
• メーカー／製品名: Cytiva 29-0491-04
• 利用可能性: not available in North America
• 保管条件: (20% Ethanol); (20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0)
• パラメーター: 42 psi
• ベッドサイズ: 7 mm × 25 mm
• ベッド容積: 1 mL
• カラム内径: 7 mm
• Matrix: highly cross-linked agarose
• 平均部品サイズ: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-13.7
• 動作範囲: 3-12
• キャパシティ: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• 適合性: suitable for bioprocess medium
• 保管温度: 2-8°C

詳細

詳細

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allows cleaning-in-place (CIP).

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特徴および利点

• Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
• Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.

HiTrap is a registered trademark of Cytiva

MabSelect SuRe is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS02
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids