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物質
bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)
分子量
10000-600000 Da (globular proteins)
包装
pkg of 1 ea
メーカー/製品名
Cytiva 28-9893-36
パラメーター
<13 mL sample volume
<2.6 mL/min flow rate
0.3 Mpa
ベッド容積
320 mL
カラムL × 内径
60 cm × 26 mm
Matrix
agarose-dextran composite
dextran-agarose particle platform
polymeric
マトリックス活性基
dextran-agarose phase
dextran-agarose phase
平均部品サイズ
34 μm
cleaning
1-14
動作範囲
3-12
分離法
size exclusion (SEC)
詳細
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
特徴および利点
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
適合性
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
プロトコル
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.
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