GE28-9545-49

pGEX-4T-1

Cytiva 28-9545-49

• UNSPSCコード: 41106501
• NACRES: NA.56

特性

• 包装: pkg of 25 μg
• メーカー／製品名: Cytiva 28-9545-49
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

Thirteen pGEX vectors are available (see Figure). Nine of the vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease, (see PreScission Protease) between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

GEX-2TK is designed to allow the detection of expressed proteins by directly labeling the fusion products in vitro (1). This vector contains the recognition sequence for the catalytic subunit of cAMP-dependent protein kinase obtained from heart muscle. The protein kinase site is located between the GST domain and the MCS. Expressed proteins can be directly labeled using protein kinase and [gamma-32P]ATP and readily detected using standard radiometric or autoradiographic techniques. pGEX-2TK is a derivative of pGEX-2T; its fusion proteins can be cleaved with Thrombin.

Cleavage of pGEX-6P GST fusion proteins occurs between the Gln and Gly residues of the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro (2). Low temperature (5°C) digestion minimizes the degradation of the protein of interest. Because PreScission Protease has been engineered with a GST tag, it can also be removed from the cleavage mixture simultaneously with the GST portion of the fusion protein. The pGEX-6P Expression Vectors permit convenient site-specific cleavage and simultaneous purification on Glutathione Sepharose. The pGEX-6P series provides all three translational reading frames linked between the GST coding region and the multiple cloning site.

Collectively, the pGEX vectors provide all three translational reading frames beginning with the EcoRI restriction site. pGEX-1λT, pGEX-6P-1, pGEX-4T-1, and pGEX-5X-1 can directly accept and express cDNA inserts isolated from λgt11 libraries.

特徴および利点

• 化学的に誘導可能な、高レベルでの GST タグ付き組み換えタンパク質の発現のための Tac プロモーター
• いずれの大腸菌宿主
でも使用可能な内在性 laclq 遺伝子
アフィニティーマトリクスからの融合タンパク質の放出は非常に穏やかな溶出条件下でおこなわれるため、抗原性や機能的活性への影響は最小限に抑えられます。融合生成物から目的のタンパク質を切り出すための
• PreScission^™ Protease、トロンビンまたは第 Xa 因子の認識部位。

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

本製品の分析証明書を参照するにはwww.cytiva.comにアクセスしてください。

法的情報

pGEX ベクター
pGEX ベクターは、科学的な調査および研究に使用されるべきものであり、他の目的には一切使用すべきものではなく、米国特許第 5,654,176 号ならびに米国以外の国におけるこれと等価の特許および特許出願においてライセンスを受けた製品およびプロセスの、商用利用に対するライセンスについては、購入者がこれらの用途に使用する前に、ミリポア社（旧名称、ケミコンインターナショナル）と直接協議しなければなりません。

PreScission is a trademark of Cytiva

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1