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包装
pkg of 1 ea
メーカー/製品名
Cytiva 28-9365-52
パラメーター
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
ベッド容積
20 mL
カラム内径
16 mm
マトリックス
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
2-14(Medium uncharged with metal ions.)
working range
3-12
キャパシティ
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
適合性
suitable for bioprocess medium
関連するカテゴリー
詳細
アプリケーション
IMAC Sepharose™ 6 Fast FLow consists of 90 ?m beads of highly cross-linked Agarose to which a chelating group has been covalently coupled. This chelating group can be charged with suitable metal ions, specific for your target Protein of interest. The bead size of IMAC Sepharose™ 6 Fast FLow enables high flow properties, making the medium an excellent choice for scaling up purification at laboratory scale and purification of histidine-tagged Proteins at process scale. Low metal ion leakage means that the activity of the purified Protein is retained and the risk of precipitation reduced, which results in increased purity, activity, and yield of the target Protein. Leakage of metal ions into the eluted target Protein pool from IMAC Sepharose™ 6 Fast FLow is generally Low under normal conditions.
IMAC Sepharose™ 6 Fast FLow is highly stable and compatible with a wide range of common additives. This helps maintain biological activity and increase product yield, while at the same time greatly expanding the range of suitable operating conditions.
The medium is available prepacked in HiTrap® HiScreen™ and HiPrep™ column formats for convenient purification of histidine-tagged Proteins at laboratory scale. Initial purification using a 1 mL HiTrap® IMAC FF column can be scaled up further with the 5 mL column. Purification can be scaled up even more using 20 mL HiPrep™ IMAC FF 16/10 columns. Prepacked HiScreen™ IMAC FF with a 10 cm bed height is the format of choice for method optimization and process development.
特徴および利点
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion.
- Conveniently charge with your metal of choice.
- Prepacked 20 mL HiPrep™ columns for easy scale-up.
- High binding capacity.
保管および安定性
アナリシスノート
その他情報
法的情報
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
プロトコル
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
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