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物質
polycarbonate housing
polyethersulfone membrane
無菌性
non-sterile
特徴
wetted part: no
包装
pkg of 25 ea
メーカー/製品名
Cytiva 28932293
チューブサイズ
15 mL
有効容積
6 mL
ポアサイズ
3 kDa MWCO
詳細
Vivaspin ultrafiltration spin columns are designed for fast, non-denaturing concentration and desalting of proteins and other biological samples. Membrane spin columns such as Vivaspin combine the principle of size exclusion with centrifugation to achieve rapid concentration and high recovery of target biomolecules in a single tube. An upper compartment containing sample is separated from the lower compartment by a semipermeable membrane with an appropriate MWCO value chosen to exclude molecules of interest. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber. The Vivaspin column design permits protocols for one-step biomolecule concentration, rapid sample recovery (reverse spin protocol), desalting, and buffer exchange.
特徴および利点
- Ease-of-use: One-step sample concentration in a single tube, for minimal sample handling and reduced sample loss, as well as pH compatibility over a broad range – pH 1 to 9.
- Sample protection: Patented Vivaspin dead-stop technology ensures samples cannot be concentrated to dryness and enables direct concentrate recovery.
- Ease-of-use: One-step sample concentration in a single tube, for minimal sample handling and reduced sample loss.
- High flow rates: And minimal membrane blockage through the vertical polyethersulfone (PES) membrane design.
- Minimal protein adsorption: Thanks to the Vivaspin™ polycarbonate tube design.
- Wide selection: Vivaspin sample concentrators are suitable for sample volumes ranging from 100 μL to 20 mL, with a range of molecular weight cut-off (MWCO) values from Mr 3000 to 100 000.
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()
保管分類コード
13 - Non Combustible Solids
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資料
This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.
プロトコル
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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