GE28-9187-80

MBPTrap^™ HP

Cytiva 28-9187-80, pack of 5 × 5 mL

• 別名: MBP Trap
• UNSPSCコード: 23151817
• NACRES: NA.56

特性

• ligand: dextrin
• 包装: pack of 5 × 5 mL
• メーカー／製品名: Cytiva 28-9187-80
• 保管条件: (20% Ehtanol)
• パラメーター: <20 mL/min flow rate; 42 psi
• ベッドサイズ: 16 mm × 25 mm
• ベッド容積: 5 mL
• カラム内径: 16 mm
• Matrix: highly cross-linked 6% agarose
• 平均部品サイズ: 34 μm
• cleaning: 2-13
• 動作範囲: >7
• キャパシティ: ~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)
• 保管温度: 2-8°C

詳細

詳細

MBPTrap^™ HP columns prepacked with Dextrin Sepharose^™ High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP). This robust and stable Sepharose High Performance based resin enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps.

Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

特徴および利点

• HiTrap^® columns prepacked with Dextrin Sepharose^™ High Performance medium.
• Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
• Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
• Physiological conditions and mild elution preserve the activity of the target protein.
• Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

HiTrap is a registered trademark of Cytiva

MBPTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS02,GHS02
• シグナルワード: WarningDanger
• 危険有害性情報: H226 - H225
• 注意書き: P210 - P235 - P241 - P280 - P210 - P233 - P240 - P241 - P242 - P243 - P303 + P361 + P353 - P501
• 危険有害性の分類: Flam. Liq. 2
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 1