コンテンツへスキップ
Merck
すべての画像(1)

Key Documents

GE28-9031-34

Protein G High Performance Spintrap

Cytiva 28-9031-34, pack of 16 columns

別名:

Protein G Column

ログイン組織・契約価格を表示する


About This Item

UNSPSCコード:
41106500
NACRES:
NA.25

ligand

recombinant protein G lacking albumin-binding region

特徴

wetted part: no

包装

pack of 16 columns

メーカー/製品名

Cytiva 28-9031-34

保管条件

(20% Ehtanol)
(20% Ethanol)

マトリックス

highly cross-linked 6% agarose

平均部品サイズ

34 μm

cleaning

2-9

working range

3-9

キャパシティ

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
>1 mg binding capacity(human IgG/column)

保管温度

2-8°C

詳細

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
protein G HP SpinTrap is a prepacked, single-use spin column containing protein G Sepharose High Performance.

アプリケーション

Protein G Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).


There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

特徴および利点

  • For simple, small-scale purification of antibodies or enrichment of specific Proteins in prepacked spin columns that are ready to use.
  • Classic and cross-link protocols provide flexibility.
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provides high yield.
  • Prepacked with Protein G Sepharose High Performance for coupling of antibodies of IgG subclasses.
  • Easy scale-up with HiTrap® Protein G HP prepacked columns.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

資料

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

This page describes immunoprecipitation (immunoaffinity or pull-down techniques) with GE Healthcare products.

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

プロトコル

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from GE Healthcare.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from GE Healthcare.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら(テクニカルサービス)