GE28-9031-32

Protein A High Performance Spintrap^™

Cytiva 28-9031-32, pack of 16 columns

• 別名: High Performance Spintrap, Protein A Spintrap
• UNSPSCコード: 41106500
• NACRES: NA.25

特性

• ligand: native protein A
• 特徴: wetted part: no
• 包装: pack of 16 columns
• メーカー／製品名: Cytiva 28-9031-32
• 保管条件: (20% Ethanol)
• Matrix: highly cross-linked 6% agarose
• 平均部品サイズ: 34 μm
• cleaning: 2-10
• 動作範囲: 3-9
• キャパシティ: >1 mg binding capacity(human IgG well)
• 保管温度: 2-8°C

詳細

詳細

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

protein A HP SpinTrap^™ are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose^™ HP.

アプリケーション

Protein A Sepharose^™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

特徴および利点

• For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use.
• Classic and cross-link protocols provide flexibility.
• Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
• Fast binding kinetics and high capacity provide high yield.
• Prepacked with Protein A Sepharose^™ High Performance for coupling of antibodies of IgG subclasses.
• Easy scale-up with HiTrap^® Protein A HP prepacked columns.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

SpinTrap is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS02
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids