GE28-4009-90

His MultiTrap^™ Fast Flow

Cytiva 28-4009-90, pack of 4 plates

• 別名: His Tag Affinity Purification
• UNSPSCコード: 12161501
• NACRES: NA.32

特性

• 物質: polypropylene and polyethylene
• 包装: pack of 4 plates
• メーカー／製品名: Cytiva 28-4009-90
• サイズ: 127.8 mm × 85.5 mm × 30.6 mm
• マトリックス: 6% cross-linked agarose
• 粒径: 45-165 μm
• 平均直径: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• working range: 3-12(Ni²⁺-stripped medium.)
• キャパシティ: ~40 mg binding capacity(histidine-tagged protein)
• 適合性: suitable for bioprocess medium

詳細

詳細

His MultiTrap^™ FF and His MultiTrap^™ HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC).

アプリケーション

His MultiTrap^™ FF and His MultiTrap^™ HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* Proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose^™ 6 Fast FLow (His MultiTrap^™ FF) or Ni Sepharose^™ High Performance (His MultiTrap^™ HP). A protocol for His MultiTrap^™ is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation.

Loading unclarified samples directly to His MultiTrap^™ FF and His MultiTrap^™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap^™ FF and His MultiTrap^™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.

特徴および利点

• Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins.
• Increased convenience and consistency with prepacked 96-well plates.
• No filtration needed - load unclarified sample directly, increase reproducibility, and save time.
• Use His MultiTrap^™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
• Easy scale-up to HisTrap^™ FF, HisPrep^™ FF 16/10 or HisTrap^™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

HisPrep is a trademark of Cytiva

HisTrap is a trademark of Cytiva

MultiTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS09
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids