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包装
pack of 1 kit
メーカー/製品名
Cytiva 28-9321-71
保管条件
(0.15% Kathon CG)
(20% Ethanol)
Matrix
highly cross-linked 6% agarose
平均部品サイズ
34 μm
cleaning
2-14(Ni2+-stripped medium.)
動作範囲
3-12(Ni2+-stripped medium.)
キャパシティ
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
保管温度
2-8°C
関連するカテゴリー
詳細
特徴および利点
- For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
- Ni Sepharose High Performance in prepacked spin columns.
- Yields up to 750 µg pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates.
構成
1 pack - GE28-4013-53 - His SpinTrap™
1 pack - GE11-0034-00 - His Buffer Kit
保管および安定性
アナリシスノート
法的情報
キットの構成要素は別途購入可能です。
- GE11-0034-00His Buffer Kit, Cytiva 11-0034-00SDS
- GE28-4013-53His Spintrap™, Cytiva 28-4013-53SDS
シグナルワード
Danger
危険有害性情報
保管分類コード
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
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資料
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
プロトコル
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose High Performance from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose High Performance from GE Healthcare.
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