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Merck

GE17-6003-77

Immobiline® Drystrip pH 3-11 NL, 24 cm

Cytiva 17-6003-77, pack of 12 strips

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About This Item

UNSPSCコード:
41105323
NACRES:
NA.25

形状

solid

特徴

wetted part: no

包装

pack of 12 strips

メーカー/製品名

Cytiva 17-6003-77

Colorless

溶解性

cold water: soluble (Insoluble in cold and hot water)

輸送温度

dry ice

保管温度

−20°C

詳細

Immobiline® DryStrip gels (IPG strips) are used for isoelectric focusing (IEF), run as the first dimension of 2-D electrophoresis or as a separate application.

アプリケーション

Immobiline® DryStrip gels are available in fourteen overlapping pH ranges and five strip lengths (7, 11, 13, 18, and 24 cm) and represent the most comprehensive selection of narrow-, medium-, and wide-range IPG strips available. The five narrow pH range (1 pH unit), 24-cm IPG strips deliver the highest resolution and Protein loading capacity available, which results in well separated spots with high Protein content after the second-dimension separation.

The pH 3–11 NL strip covers most Proteins found in prokaryotic and eukaryotic cells while the narrow-range strips can be used when higher resolution is required. The pH gradients at the extreme ends of the pH scale are nonlinear to distribute the Proteins evenly over the gel length to obtain maximal resolution.

Immobiline® DryStrip gels are rehydrated with a matching IPG Buffer prior to use. Samples can be included directly in the rehydration solution for efficient loading of dilute samples or for loading larger amounts of Protein. Samples can also be loaded after rehydration. The first-dimension separation is carried out using Ettan IPGphor 3 IEF system or Multiphor II flatbed system.

特徴および利点

  • Maximize resolution, loading capacity, and reproducibility of first-dimension isoelectric focusing (IEF).
  • Choose from a comprehensive range of overlapping Immobiline® DryStrip gels that cover narrow (1 pH unit), medium (3–5 pH units), and broad (8 pH units) pH ranges.
  • Select the broad pH 3–11 NL gradient Immobiline® DryStrip gel for fast and efficient screening to gain a broad overview of total Protein distribution.
  • For more detailed studies or when using greater Protein loads, choose from four new overlapping strips covering the pH 3 to 11 range.
  • Higher resolution can be obtained using narrow-range (1 pH unit) Immobiline® DryStrip gels.
  • Use matching IPG buffers for optimized running conditions.
  • Use IPG strips directly after reswelling without further manipulation.
  • Barcoded strips for convenient sample and batch tracking.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

Ettan is a trademark of Cytiva
Immobiline is a registered trademark of Cytiva

保管分類コード

13 - Non Combustible Solids

WGK

WGK 2


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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資料

This page describes troubleshooting second-dimension SDS-PAGE with Multiphor™ II Electrophoresis System.

プロトコル

This page describes troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from GE Healthcare.

Rehydration Solutions

Rehydration Solutions

This page describes two-dimensional separation of protein samples with GE Healthcare products.

関連コンテンツ

This page shows how to apply equilibrated Immobiline DryStrip gels

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