おすすめの製品
ligand
diethylaminoethyl
詳細
Ion Exchanger Type (value)
包装
pack of 5 × 5 mL
メーカー/製品名
Cytiva 17-5154-01
パラメーター
42 psi
ベッドサイズ
16 mm × 25 mm
ベッド容積
5 mL
カラム内径
16 mm
マトリックス
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
2-14
working range
2-12
適合性
suitable for bioprocess medium
詳細
Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached.
特徴および利点
- Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
- The industry standard for ion exchange chromatography.
- High flow rates and good scale-up potential.
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
- Predictable scale-up
保管および安定性
アナリシスノート
その他情報
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
This page shows how to perform a purification of His-tagged membrane proteins.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
プロトコル
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
Sample Preparation in Ion Exchange Chromatography
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