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Merck

GE17-5132-02

Glutathione Sepharose 4 Fast Flow

Cytiva 17-5132-02, pack of 100 mL

別名:

Sepharose 4 Fast Flow

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About This Item

UNSPSCコード:
41106500
NACRES:
NA.56

ligand

glutathione and 10-carbon linker arm

包装

pack of 100 mL

メーカー/製品名

Cytiva 17-5132-02

Matrix

highly cross-linked 4% agarose

平均直径

90 μm

cleaning

3-12

動作範囲

3-12

キャパシティ

>10 mg/mL binding capacity

関連するカテゴリー

詳細

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.

Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose 4 Fast Flow provides good flow properties and is excellent for scaling up.

特徴および利点

  • Fast, one-step purification of GST-tagged proteins
  • Mild elution conditions preserving protein antigenicity and function
  • For scale-up due to high flow properties
  • Glutathione coupled to Sepharose 4 Fast Flow via a 10-carbon spacer arm

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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Lot/Batch Number

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資料

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

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プロトコル

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.

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