おすすめの製品
ligand
IMAC immobilized with Ni
包装
pack of 10 × 1 mL
メーカー／製品名
Cytiva 17-3712-22
パラメーター
Ambient temp. range
Matrix
highly cross-linked spherical agarose including magnetite
関連するカテゴリー
詳細
His Mag Sepharose Excel are magnetic beads designed for rapid, small-scale purification and screening of his-tagged proteins consisting of an Immobilized Metal Affinity Chromatography (IMAC) resin precharged with nickel ions that are strongly bound to a chelating ligand.
Small-scale capture, purification, and screening of histidine-tagged proteins that are expressed and secreted into supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells in HyClone HyCell CHO cell culture media.
The base of the matrix is based on Sepharose resin, incorporated with magnetite. His Mag Sepharose is available as 10% slurry in three different pack sizes: 2 x 1 mL, 5 x 1 mL, and 10 x 1mL.
Our ISO 13485 accredited manufacturing center of excellence adheres to the standards in the manufacturing process, ensuring the highest quality and compliance with international medical device regulations.
Small-scale capture, purification, and screening of histidine-tagged proteins that are expressed and secreted into supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells in HyClone HyCell CHO cell culture media.
The base of the matrix is based on Sepharose resin, incorporated with magnetite. His Mag Sepharose is available as 10% slurry in three different pack sizes: 2 x 1 mL, 5 x 1 mL, and 10 x 1mL.
Our ISO 13485 accredited manufacturing center of excellence adheres to the standards in the manufacturing process, ensuring the highest quality and compliance with international medical device regulations.
特徴および利点
- Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture
- Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort
- Increase target protein yield and decrease degradation through reduced and simplified sample handling
- Screen histidine-tagged proteins in parallel with high reproducibility and reliable results
- Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale
- Simplify handling using visible and dense Sepharose-based magnetic beads
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% ethanol, 5% medium slurry)
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
Sepharose is a trademark of Cytiva
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
最新バージョンのいずれかを選択してください：
資料
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
プロトコル
This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from GE Healthcare.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from GE Healthcare.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）