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包装
pack of 100 mL
メーカー/製品名
Cytiva 17-0920-07
Matrix
highly cross-linked 6% agarose
平均部品サイズ
34 μm
cleaning in place
2-14(Medium uncharged with metal ions.)
動作範囲
3-12
キャパシティ
≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
関連するカテゴリー
詳細
アプリケーション
特徴および利点
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion
- Conveniently charge with your metal of choice
- 34 μm bead size for high performance purification
- High binding capacity
保管および安定性
アナリシスノート
法的情報
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from GE Healthcare.
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.
This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.
プロトコル
Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance
This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap Kit, HiTrap Chelating HP and Chelating Sepharose Fast Flow products from GE Healthcare.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
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