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GE17-0756-01

Glutathione Sepharose™ 4B

Cytiva 17-0756-01, pack of 10 mL

別名:

affinity chromatography resin, GST-tagged proteins purification,

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About This Item

UNSPSCコード:
41106500
NACRES:
NA.56

ligand

glutathione

包装

pack of 10 mL

メーカー／製品名

Cytiva 17-0756-01

Matrix

4% agarose

粒径

45-165 μm

平均直径

90 μm

cleaning in place

4-13

動作範囲

4-13

キャパシティ

>25 mg binding capacity (horse liver GST/ml medium)

関連するカテゴリー

詳細

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

特徴および利点

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

Sepharose is a trademark of Cytiva

ピクトグラム

Flame
GHS02

シグナルワード

Warning

危険有害性情報

H226

保管分類コード

3 - Flammable liquids

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Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 資料 section.

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

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Xin Zhou et al.
Nature communications, 11(1), 2674-2674 (2020-05-31)
Increasing evidence indicates that guidance molecules used during development for cellular and axonal navigation also play roles in synapse maturation and homeostasis. In C. elegans the netrin receptor UNC-40/DCC controls the growth of dendritic-like muscle cell extensions towards motoneurons and
Amandine Cartier-Michaud et al.
PLoS genetics, 13(6), e1006803-e1006803 (2017-06-16)
Spermatogenesis is a dynamic process that is regulated by adhesive interactions between germ and Sertoli cells. Germ cells express the Junctional Adhesion Molecule-C (JAM-C, encoded by Jam3), which localizes to germ/Sertoli cell contacts. JAM-C is involved in germ cell polarity
Ye Guan et al.
eLife, 7 (2018-04-06)
Single-cell analysis is pivotal to deciphering complex phenomena like heterogeneity, bistability, and asynchronous oscillations, where a population ensemble cannot represent individual behaviors. Bulk cell-free systems, despite having unique advantages of manipulation and characterization of biochemical networks, lack the essential single-cell
Amandine Draime et al.
FEBS letters, 592(7), 1185-1201 (2018-02-22)
HOXA1 belongs to the HOX family of transcription factors which are key regulators of animal development. Little is known about the molecular pathways controlling HOXA1. Recent data from our group revealed distinct partner proteins interacting with HOXA1. Among them, OGT
Amandine Draime et al.
Biochimica et biophysica acta, 1861(5), 534-542 (2018-02-23)
Understanding how the activity of transcription factors like HOX proteins is regulated remains a widely open question. In a recent screen for proteins interacting with HOXA1, we identified a PRDM protein family member, PRDM14, which is known to be transiently
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資料

Troubleshooting of Problems Associated with the Expression and Growth of GST-tagged Proteins

This page addresses common problems associated with the expression and growth of GST-tagged proteins using GE Healthcare products.

Purification Using GST SpinTrap

This page shows how to purify GST-tagged proteins using GST SpinTrap from GE Healthcare.

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

General Considerations for Purification of GST-tagged Protein

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

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プロトコル

GST Detection Module with CDNB Enzymatic Assay

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from GE Healthcare.

GST Detection Module with CDNB Enzymatic Assay

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from GE Healthcare.

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.

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