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Key Documents

GE17-0729-10

SP Sepharose Fast Flow

Cytiva 17-0729-10, pack of 25 mL

別名:

SP Sepharose Column, Sepharose Fast Flow

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About This Item

UNSPSCコード:
41106500
NACRES:
NA.56

ligand

sulphopropyl

詳細

Ion Exchanger Type (value)

包装

pack of 25 mL

メーカー/製品名

Cytiva 17-0729-10

マトリックス

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning in place

3-14

working range

4-13

キャパシティ

70 mg binding capacity(ribonuclease A/ml medium)

適合性

suitable for bioprocess medium

詳細

SP Sepharose Fast FLow is a strong cation exchanger based on the well established Sepharose Fast FLow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

アプリケーション

SP Sepharose Fast FLow is part of the Sepharose Fast FLow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% Agarose beads, with sulphopropyl (Q) strong cation exchange groups. SP Sepharose Fast FLow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with SP Sepharose Fast FLow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, SP Sepharose Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

特徴および利点

  • Well-proven strong cation exchanger developed for industrial downstream processes.
  • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
  • The industry standard for ion exchange chromatography during recent decades
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

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資料

Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.

Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

すべて表示

プロトコル

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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