おすすめの製品
ligand
carboxymethyl
詳細
Ion Exchanger Type (value)
包装
pack of 500 mL
メーカー/製品名
Cytiva 17-0719-01
Matrix
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
2-14
動作範囲
4-13
適合性
suitable for bioprocess medium
詳細
CM Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups. CM Sepharose™ Fast Flow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with CM Sepharose™ Fast Flow is straight forward and the medium is available in a range of pre-packed process development tools.
As member of the BioProcess media range, CM Sepharose™ Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
特徴および利点
- Well-proven weak cation exchanger developed for industrial downstream processes
- Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
- The industry standard for ion exchange chromatography during recent decades
- High chemical stability allows for well proven CIP and sanitization protocols
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
プロトコル
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
Sample Preparation in Ion Exchange Chromatography
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