おすすめの製品
ligand
calmodulin
包装
pack of 10 mL
メーカー/製品名
Cytiva 17-0529-01
保管条件
(20% Ehtanol)
マトリックス
4% agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
4-9
working range
4-9
保管温度
2-8°C
詳細
Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD?/NADP? Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca2+ on separate Ca2+-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca2+ complexes are particularly stable.
特徴および利点
- For single-step purification of native calmodulin-binding proteins
- Suitable for tandem affinity purification (TAP) of protein complexes
- Purification of calmodulin-regulated proteins from all eukaryotic cells
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
プロトコル
This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from GE Healthcare.
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
関連コンテンツ
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
アフィニティーまたはGSTプルダウン法、タンデムアフィニティー精製(TAP)法、共免疫沈降法を用いてin vitroのタンパク質間相互作用を検討するためのプルダウンアッセイ、試薬、プロトコル
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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