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ligand
concanavalin A (con A)
包装
pack of 5 mL
メーカー/製品名
Cytiva 17-0440-03
保管条件
(0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)
Matrix
4% agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
4-9
動作範囲
4-6
保管温度
2-8°C
詳細
Con A Sepharose™ 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose.
アプリケーション
Con A Sepharose™ is Concanavalin A coupled to Sepharose™ 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloProtein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose™ is routinely used for separation and purification of glycoProteins, polysaccharides and glycolipids
特徴および利点
- Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
- Group-specific adsorbent for molecules containing sugars.
- Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes
- Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
- Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes
- Con A coupled to Sepharose™ 4B via cyanogen bromide activation
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
Sepharose is a trademark of Cytiva
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
プロトコル
Purification or Removal of Glycoproteins and Polysaccharides
Purification or Removal of Glycoproteins and Polysaccharides
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
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