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Merck

GE17-0409-03

HiTrap® Chelating High Performance

Cytiva 17-0409-03, pack of 5 × 5 mL

別名:

HiTrap Chelating Column

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About This Item

UNSPSCコード:
23151816
NACRES:
NA.56

ligand

iminodiacetic acid

包装

pack of 5 × 5 mL

メーカー/製品名

Cytiva 17-0409-03

パラメーター

<20 mL/min flow rate
42 psi

ベッドサイズ

16 mm × 25 mm

ベッド容積

5 mL

カラム内径

16 mm

サイズ

平均部品サイズ

34 μm

cleaning

2-14

動作範囲

3-13

詳細

HiTrap® Chelating HP is prepacked with Chelating Sepharose High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTAdesign or FPLC Systems.

特徴および利点

  • For optimizing purification of histidine-tagged proteins using different kinds of metal ions.
  • Conveniently charge with your metal of choice.
  • Iminidoacetic acid coupled to Sepharose High Performance.
  • Purifying proteins with exposed histidine groups via metal ion complex formation.
  • Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC System.
  • Packed with Chelating Sepharose High Performance
  • Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC System

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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試験成績書(COA)

Lot/Batch Number

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資料

This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from GE Healthcare.

This page shows how to perform a purification and homogeneity check of membrane proteins with products from GE Healthcare.

This page shows how to perform a purification and homogeneity check of membrane proteins with products from GE Healthcare.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

プロトコル

This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap Kit, HiTrap Chelating HP and Chelating Sepharose Fast Flow products from GE Healthcare.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

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製品に関するお問い合わせはこちら(テクニカルサービス)