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ligand
iminodiacetic acid
包装
pack of 5 × 5 mL
メーカー/製品名
Cytiva 17-0409-03
パラメーター
<20 mL/min flow rate
42 psi
ベッドサイズ
16 mm × 25 mm
ベッド容積
5 mL
カラム内径
16 mm
サイズ
平均部品サイズ
34 μm
cleaning
2-14
動作範囲
3-13
詳細
HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTAdesign or FPLC™ Systems.
特徴および利点
- For optimizing purification of histidine-tagged proteins using different kinds of metal ions.
- Conveniently charge with your metal of choice.
- Iminidoacetic acid coupled to Sepharose™ High Performance.
- Purifying proteins with exposed histidine groups via metal ion complex formation.
- Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC™ System.
- Packed with Chelating Sepharose™ High Performance
- Simple operation with a syringe, pump or chromatographic system such as AKTA design or FPLC™ System
保管および安定性
アナリシスノート
その他情報
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from GE Healthcare.
This page shows how to perform a purification and homogeneity check of membrane proteins with products from GE Healthcare.
This page shows how to perform a purification and homogeneity check of membrane proteins with products from GE Healthcare.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
プロトコル
This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap Kit, HiTrap Chelating HP and Chelating Sepharose Fast Flow products from GE Healthcare.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
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