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Key Documents

GE17-0404-03

HiTrap® Protein G High Performance

Cytiva 17-0404-03, pack of 2 × 1 mL

別名:

HiTrap Protein G, Protein G affinity column

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About This Item

UNSPSCコード:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

形状

prepacked column

クロスリンク

cross-linked

包装

pack of 2 × 1 mL

メーカー/製品名

Cytiva 17-0404-03

保管条件

(20% Ehtanol)
(20% Ethanol)

パラメーター

42 psi

テクニック

liquid chromatography (LC): suitable

ベッドサイズ

7 mm × 25 mm

ベッド容積

1 mL

カラム内径

7 mm

サイズ

マトリックス

(cross-linked agarose, spherical)

マトリックス活性基

Protein G

平均部品サイズ

34 μm

cleaning

2-9

working range

3-9

キャパシティ

>1 mg binding capacity (lgG/column)
25 mg binding capacity(human IgG/column)

分離法

affinity

保管温度

2-8°C

詳細

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特徴および利点

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Nimitha R Mathew et al.
Cell reports, 35(12), 109286-109286 (2021-06-24)
B cell responses are critical for antiviral immunity. However, a comprehensive picture of antigen-specific B cell differentiation, clonal proliferation, and dynamics in different organs after infection is lacking. Here, by combining single-cell RNA and B cell receptor (BCR) sequencing of

資料

This page describes automated purification of antibodies using ÄKTA chromatography systems.

This page describes automated purification of antibodies using ÄKTA chromatography systems.

This page shows how to perform a separation with the HiTrap Protein G HP from GE Healthcare, an Affinity Exclusion Chromatography product containing pre-packed Protein G.

This page shows how to perform a separation with the HiTrap Protein G HP from GE Healthcare, an Affinity Exclusion Chromatography product containing pre-packed Protein G.

すべて表示

プロトコル

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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